DATASETS

Raw data derived from metagenomic sequencing:
A. Sequenced with PacBio e Ilumina:

Metagenome

Description

Accession Illumina

Accession PacBio

Citation

Med-OCT2021-15m

Metagenomic sample was collected at 15 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2021, when the water column is strongly stratified.

SRR31420722

SRR28395891

https://doi.org/10.1128/mbio.03455-24

Med-OCT2021-75m

Metagenomic sample was collected at 75 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2021, when the water column is strongly stratified.

SRR31420721

SRR28395889

https://doi.org/10.1128/mbio.03455-24

Med-SEP2022-60m

Metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2022, when the water column is strongly stratified.

SRR31420720

SRR28395890

https://doi.org/10.1128/mbio.03455-24

MedWinter-FEB2022

Metagenomic sample was collected at 20 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in February 2022, when the water column is fully mixed.

SRR31420719

SRR28395892

https://doi.org/10.1128/mbio.03455-24

MedWinter-JAN2019

Metagenomic sample was collected at 20 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in January 2019, when the water column is fully mixed.

SRR13009789

SRR13009787

https://doi.org/10.3389/fmicb.2021.708782

A. Sequenced only with Illumina:

Metagenome

Description

Accession Illumina

Citation

MedVir-OCT2015-15m

Virome was obtained from the viral fraction (< 0.22 microns) at 15 m below sea surface in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015.

SRR7638330

https://doi.org/10.1128/msystems.00554-19

MedVir-OCT2015-45m

Virome was obtained from the viral fraction (< 0.22 microns) at 45 m below sea surface in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015.

SRR7638331

https://doi.org/10.1128/msystems.00554-19

MedVir-OCT2015-60m

Virome was obtained from the viral fraction (< 0.22 microns) at 60 m below sea surface in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015.

SRR7638328

https://doi.org/10.1128/msystems.00554-19

MedVir-OCT2015-2000m

Virome was obtained from the viral fraction (< 0.22 microns) at 2000 m below sea surface in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015.

SRR7638329

https://doi.org/10.1128/msystems.00554-19

Med-OCT2015-15m

Metagenomic sample was collected at 15 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007106

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-30m

Metagenomic sample was collected at 30 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007114

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-45m

Metagenomic sample was collected at 45 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007115

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-60m

Metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007118

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-75m

Metagenomic sample was collected at 75 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007138

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-90m

Metagenomic sample was collected at 90 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007139

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-1000m

Metagenomic sample was collected at 1000 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007141

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-2000m

Metagenomic sample was collected at 2000 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified.

SRR5007147

https://doi.org/10.1186/s40168-018-0513-5

Med-OCT2015-15m-7h

Metagenomic sample was collected at 15 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified, and after a post-confinement of 7 hours on deck.

SRR8503606

https://doi.org/10.3389/fmicb.2019.02926

Med-OCT2015-15m-14h

Metagenomic sample was collected at 15 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified, and after a post-confinement of 14 hours on deck.

SRR8503605

https://doi.org/10.3389/fmicb.2019.02926

Med-OCT2015-60m-7h

Metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified, and after a post-confinement of 7 hours on deck.

SRR8503608

https://doi.org/10.3389/fmicb.2019.02926

Med-OCT2015-60m-14h

Metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified, and after a post-confinement of 14 hours on deck.

SRR8503607

https://doi.org/10.3389/fmicb.2019.02926

Med-OCT2015-90m-7h

Metagenomic sample was collected at 90 m deep in the epipelagic Mediterranean Sea at 60 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2015, when the water column is strongly stratified, and after a post-confinement of 7 hours on deck.

SRR8503604

https://doi.org/10.3389/fmicb.2019.02926

MedWinter-JAN2015-20m

Metagenomic sample was collected at 20 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in January 2015, when the water column is fully mixed.

SRR3405540

https://doi.org/10.1186/s40168-018-0513-5

MedWinter-JAN2015-20m-LF

Metagenomic sample was collected at 20 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in January 2015, when the water column is fully mixed. The sample corresponds to the particulate fraction (5-20 micron filter).

SRR3407091

https://doi.org/10.1186/s40168-018-0513-5

MedWinter-JAN2015-80m

Metagenomic sample was collected at 80 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in January 2015, when the water column is fully mixed.

SRR5877433

https://doi.org/10.1186/s40168-018-0513-5

Med-SEP2014-15m

Metagenomic sample was collected at 15 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2014, when the water column is strongly stratified.

SRR5877534

https://doi.org/10.1371/journal.pgen.1007018

Med-SEP2014-30m

Metagenomic sample was collected at 30 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2014, when the water column is strongly stratified.

SRR5877535

https://doi.org/10.1371/journal.pgen.1007018

MedDCM-SEP2014-60m

Metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2014, when the water column is strongly stratified.

SRR3993157

https://doi.org/10.1371/journal.pgen.1007018

MedDCM-SEP2015_HS

Metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2015, when the water column is strongly stratified.

SRR5010551

https://doi.org/10.1371/journal.pgen.1007018

MedWinter-DEC2013-20m

Metagenomic sample was collected at 20 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in December 2013, when the water column is fully mixed.

SRR5877432

https://doi.org/10.1371/journal.pgen.1007018

MedDCM-SEP2013

Metagenomic sample was collected at 55 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2013, when the water column is strongly stratified.

SRR1539645

https://doi.org/10.1371/journal.pgen.1007018

MedDCM-SEP2013-LF

Metagenomic sample was collected at 60 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2015, when the water column is strongly stratified. The sample corresponds to the particulate fraction (5-20 micron filter).

SRR1539385

https://doi.org/10.1371/journal.pgen.1007018

MedDCM-JUL2012

Metagenomic sample was collected at 75 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in September 2013, when the water column is strongly stratified.

SRR1539383

https://doi.org/10.1371/journal.pgen.1007018

B. Metagenome-assembled genomes (MAGs) recovered from metagenomic samples:

Description

Number of MAGs

Bioproject Accession Number

Citation

MAGs encompassing multiple taxa from metagenomic binning of samples Med-OCT2021-15m, Med-OCT2021-75m, Med-SEP2022-60m, and MedWinter-FEB2022. MAG pool starts from LRMED-001 and ends in LRMED-431.

418

PRJNA1088973

UNPUBLISHED

MAGs encompassing multiple taxa from metagenomic binning of sample MedWinter-JAN2019. MAG pool starts from PBMED-G01 and ends in PBMED-G77.

77

PRJNA674982

https://doi.org/10.3389/fmicb.2021.7087

MAGs encompassing multiple taxa from metagenomic binning of samples Med-OCT2015-15m, Med-OCT2015-30m, Med-OCT2015-45m, Med-OCT2015-60m, Med-OCT2015-75m, Med-OCT2015-90m, MedWinter-JAN2015-20m, and MedWinter-JAN2015-80m. MAG pool starts from MED-G01 and ends in MED-G94.

94

PRJNA352798

https://doi.org/10.1186/s40168-018-0513-5

MAGs encompassing multiple taxa from metagenomic binning of samples Med-OCT2015-15m-7h, Med-OCT2015-15m-14h, Med-OCT2015-60m-7h, Med-OCT2015-60m-14h, and Med-OCT2015-90m-7h. MAG pool starts from MED-G95 and ends in MED-G188.

94

PRJNA352798

https://doi.org/10.3389/fmicb.2019.02926

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